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. 2010 Sep-Oct;1(5):386-90.
doi: 10.4161/viru.1.5.12486.

Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome

Judy A Mikovits  1 , Vincent C Lombardi, Max A Pfost, Kathryn S Hagen, Francis W Ruscetti
Affiliations

Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome

Judy A Mikovits et al. Virulence. 2010 Sep-Oct.

Abstract

In October 2009, we reported the first direct isolation of infectious xenotropic murine leukemia virus-related virus (XMRV). In that study, we used a combination of biological amplification and molecular enhancement techniques to detect XMRV in more than 75% of 101 patients with chronic fatigue syndrome (CFS). Since our report, controversy arose after the publication of several studies that failed to detect XMRV infection in their CFS patient populations. In this addenda, we further detail the multiple detection methods we used in order to observe XMRV infection in our CFS cohort. Our results indicate that PCR from DNA of unstimulated peripheral blood mononuclear cells is the least sensitive method for detection of XMRV in subjects' blood. We advocate the use of more than one type of assay in order to determine the frequency of XMRV infection in patient cohorts in future studies of the relevance of XMRV to human disease.

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References

    1. Fukuda K, Straus S, Hickie I, Sharpe MC, Dobbins JG, Komaroff A. The chronic fatigue syndrome: A comprehensive approach to its definition and study. Ann Intern Med. 1994;121:953–959. - PubMed
    1. Carruthers B, Jain A, DeMeirlier K, Peterson DL, Klimas NG, Lerner AM, et al. Myalgic encephalomyelitis/chronic fatigue syndrome: Clinical working case definition. diagnostic and treatment protocols. J Chronic Fatigue Syndrome. 2003;11:1–12.
    1. Jason L, Torres-Harding S, Jurgens A, Helgerson J. Comparing the Fukuda et al. criteria and the Canadian definition for chronic fatigue syndrome. J Chronic Fatigue S. 2004;12:37–52.
    1. Erlwein O, Kaye S, McClure MO, Weber J, Wills G, Collier D, et al. Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome. PLoS One. 5:8519. - PMC - PubMed
    1. Groom HC, Boucherit VC, Makinson K, Randal E, Baptista S, Hagan S, et al. Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome. Retrovirology. 7 - PMC - PubMed

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