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. 2011 Mar 7;47(9):2571-3.
doi: 10.1039/c0cc04292g. Epub 2010 Dec 21.

The tightly regulated copper window in yeast

Seraphine V Wegner  1 , Fei Sun, Nick Hernandez, Chuan He
Affiliations

The tightly regulated copper window in yeast

Seraphine V Wegner et al. Chem Commun (Camb). .

Abstract

The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu(+). The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.

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