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. 2011 Apr 29;1218(17):2402-4.
doi: 10.1016/j.chroma.2010.12.008. Epub 2010 Dec 13.

Dissociation and fractionation of heavy and light chains from IgG monoclonal antibodies

Pete Gagnon  1 , George Rodriquez, Simin Zaidi
Affiliations

Dissociation and fractionation of heavy and light chains from IgG monoclonal antibodies

Pete Gagnon et al. J Chromatogr A. .

Abstract

A basic method for dissociation and fractionation of monoclonal IgG heavy and light chain is described. It employs less noxious and hazardous reagents than the classical mercaptoethanol/propionic acid process and replaces size exclusion chromatography with cation exchange on a monolith to improve productivity. Significant scope remains to refine the conditions. The method can be applied to other disulfide bonded proteins with significant affinity for cation exchangers.

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