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. 2011 Mar;44(4):348-50.
doi: 10.1016/j.clinbiochem.2010.12.011. Epub 2010 Dec 23.

A simple and inexpensive automated technique for measurement of serum nitrite/nitrate

Etiane Tatsch  1 , Guilherme Vargas Bochi, Renata da Silva Pereira, Helena Kober, Vanessa Albertina Agertt, Marli Matiko Anraku de Campos, Patrícia Gomes, Marta Maria Medeiros Frescura Duarte, Rafael Noal Moresco
Affiliations

A simple and inexpensive automated technique for measurement of serum nitrite/nitrate

Etiane Tatsch et al. Clin Biochem. 2011 Mar.

Abstract

Objective: We described an automated technique for measurement of serum nitrite/nitrate (NO(x)) using the Cobas Mira clinical chemistry analyzer.

Design and methods: NO(x) was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed.

Results: The automated technique for measurement of serum NO(x) was linear, precise, and accurate. It has a LLOQ of 2.0 μmol/L.

Conclusion: Serum NO(x) measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer.

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