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. 1968 Feb;126(2):263-274.

THE USE OF COMBINED PRESERVATION TECHNIQUES FOR EXTENDED STORAGE OF ORTHOTOPIC LIVER HOMOGRAFTS

Lawrence Brettschneider  1 , Pierre M Daloze, Claude Huguet, Kenneth A Porter, Carl G Groth, Noboru Kashiwagi, David E Hutchison, Thomas E Starzl
Affiliations

THE USE OF COMBINED PRESERVATION TECHNIQUES FOR EXTENDED STORAGE OF ORTHOTOPIC LIVER HOMOGRAFTS

Lawrence Brettschneider et al. Surg Gynecol Obstet. 1968 Feb.
No abstract available

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Figures

Fig. 1
Fig. 1
Technique of orthotopic liver transplantation. The arterial supply is derived from the proximal part of the common hepatic artery by an end-to-end anastomosis to the proximal part of the recipient hepatic artery. The distal portion of the recipient artery and its inferiorly directed branches are left undisturbed. Failure to do so may result in duodenal necrosis.
Fig. 2
Fig. 2
Preservation unit. The perfusion pumps are located outside of the hyperbaric chamber. The organ receptacle, the oxygenator, and the venous reservoir are inside. The various chamber inlets permit sampling of the perfusate, gas sterilization, and oxygen delivery and removal. The temperature is electronically controlled.
Fig. 3
Fig. 3
The first 100 postoperative days of a dog which received an orthotopic liver homograft after preservation for 14½ hours. The total liver perfusion was only 3 milliliters per gram tissue per hour, a rate thought to be inadequate. Note the early evidence of reversible ischemic injury. The later rise in serum glutamic pyruvic transaminase was probably due to low grade rejection. The dog is still alive after 5½ months. This dog, as well as all others in the study, was treated with azathioprine, a short postoperative course of prednisone, and antilymphocyte globulin
Fig. 4
Fig. 4
The effect of both preservation time and perfusion rate upon circulating plasma proteins. There is profound and sustained hypoproteinemia in the dogs which received grafts that had been preserved with low flow perfusion as compared with the results using a higher flow rate.
Fig. 5
Fig. 5
The course of a dog which received an orthotopic liver homograft which had been preserved for 24¾ hours. The perfusion rate was twice that used for the experiment shown in Figure 3. There is relatively minor biochemical evidence of early ischemic injury. The dog is still alive after 4 months.
See this image and copyright information in PMC

References

    1. Ackermann JRW, Barnard CN. A report on the successful storage of kidneys. Brit J Surg. 1966;53:525. - PubMed
    1. Brown H, Patel J, Blair DW, Brown ME. Biochemical studies with preserved transplanted canine liver. J Am M Ass. 1966;196:775. - PubMed
    1. Brettschneider L, Daloze PM, Huguet C, Groth CG, Kashiwagi N, Hutchison DE, Starzl TE. Successful orthotopic transplantation of liver homografts after 8–25 hours preservation. Surgical Forum; Clinical Congress 1967. Vol. XVIII; Chicago: American College of Surgeons; 1967. p. 376. - PMC - PubMed
    1. Goodrich ED, Welsh HF, Nelson JA, Beecher TS, Welch CS. A report on homotransplantation of the canine liver. Surgery. 1956;39:244. - PubMed
    1. Kestens PJ, McDermott WV., Jr Perfusion and replacement of the canine liver. Surgery. 1961;50:196. - PubMed

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