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. 2010 Dec;42(6):392-6.
doi: 10.4103/0253-7613.71922.

The study of aqueous extract of Pterocarpus marsupium Roxb. on cytokine TNF-α in type 2 diabetic rats

Kirana Halagappa  1 , H N Girish, B P Srinivasan
Affiliations

The study of aqueous extract of Pterocarpus marsupium Roxb. on cytokine TNF-α in type 2 diabetic rats

Kirana Halagappa et al. Indian J Pharmacol. 2010 Dec.

Abstract

Objective: This study was designed to investigate the effect of aqueous extract of Pterocarpus marsupium Roxb. on elevated inflammatory cytokine, tumor necrosis factor (TNF)-α in type 2 diabetic rats.

Materials and methods: Type 2 diabetes was induced by administering streptozotocin (90 mg/kg, i.p.) in a neonatal rat model. Aqueous extract of P. marsupium at a dose of 100 and 200 mg/kg was given orally to desired group of animals for a period of 4 weeks. After 4 weeks of drug treatment, parameters such as fasting blood glucose, postprandial blood glucose, and TNF-α in serum were analyzed.

Results: Aqueous extract of P. marsupium at both doses, i.e., 100 and 200 mg/kg, decreased the fasting and postprandial blood glucose in type 2 diabetic rats. The 200 mg/kg had more pronounced effect on postprandial hyperglycemia. The drug also improved the body weight of diabetic animals. Cytokine TNF-α was found to be elevated in untreated diabetic rats due to chronic systemic inflammation. The aqueous extract at both doses significantly (P < 0.001) decreased the elevated TNF-α level in type 2 diabetic rats.

Conclusion: Modulation of cytokine TNF-α by the rasayana drug P. marsupium is related with its potential anti-diabetic activity.

Keywords: Cytokine; Pterocarpus marsupium; TNF-α; Type 2 diabetes.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Effect of aqueous extract of Pterocarpus marsupium (PM) on fasting blood glucose of type 2 diabetic rats. Values are mean ± SEM; n=6; *P < 0.05; ***P < 0.001 as compared to type 2 diabetic control; F = 165.5; df = 4, 25 (one-way ANOVA followed by Tukey-Kramer multiple comparison test); NR: Normal, DC: type 2 diabetic control, D + 100 PM: type 2 diabetic treated with 100 mg/kg of PM, D + 200 PM: type 2 diabetic treated with 200 mg/kg of PM, D + GLZ: type 2 diabetic treated with gliclazide.
Figure 2
Figure 2
Effect of aqueous extract of Pterocarpus marsupium (PM) on postprandial blood glucose of type 2 diabetic rats. Values are mean ± SEM; n = 6; *P < 0.05; ***P < 0.001 as compared to type 2 diabetic control; F = 237.3; df = 4, 25 (one-way ANOVA followed by Tukey- Kramer multiple comparison test). NR: Normal, DC: type 2 diabetic control, D + 100 PM: type 2 diabetic treated with 100 mg/kg of PM, D + 200 PM: type 2 diabetic treated with 200 mg/kg of PM, D + GLZ: type 2 diabetic treated with gliclazide.
Figure 3
Figure 3
Effect of aqueous extract of Pterocarpus marsupium (PM) on body weight of type 2 diabetic rats. Values are mean ± SEM; n=6; **P < 0.01, ***P < 0.001 as compared to type 2 diabetic control; F = 17.8; df = 4, 25 (one-way ANOVA followed by Tukey-Kramer multiple comparison test). NR: Normal, DC: type 2 diabetic control, D + 100 PM: type 2 diabetic treated with 100 mg/kg of PM, D + 200 PM: type 2 diabetic treated with 200 mg/kg of PM, D + GLZ: type 2 diabetic treated with gliclazide.
Figure 4
Figure 4
Effect of aqueous extract of Pterocarpus marsupium (PM) on TNF-α level in type 2 diabetic rats. Values are mean ± SEM; n = 6; *P < 0.05; ***P < 0.001 as compared to type 2 diabetic control; F = 32.8; df = 4, 25 (one-way ANOVA followed by Tukey-Kramer multiple comparison test). NR: Normal, DC: type 2 diabetic control, D + 100 PM: type 2 diabetic treated with 100 mg/kg of PM, D + 200 PM: type 2 diabetic treated with 200 mg/kg of PM, D + GLZ: type 2 diabetic treated with gliclazide.
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