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Review
. 1990 Sep-Nov;236(2-3):239-52.
doi: 10.1016/0921-8777(90)90008-s.

A critical review of permeabilized cell systems for studying mammalian DNA repair

S Keeney  1 , S Linn
Affiliations
Review

A critical review of permeabilized cell systems for studying mammalian DNA repair

S Keeney et al. Mutat Res. 1990 Sep-Nov.

Abstract

Permeabilized cell systems have proven valuable for studies of the enzymology of mammalian DNA repair and this review will summarize and contrast the different systems used to this end. Results from permeable cell studies will be reviewed which pertain to 3 questions of DNA repair: the role(s) of ATP, DNA polymerase enzymology, and the isolation of repair factors by in vitro correction of repair-defective cells.

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