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. 2011 Mar;49(3):1137-9.
doi: 10.1128/JCM.01831-10. Epub 2010 Dec 29.

Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method

Raquel Moure  1 , Laura Muñoz, Miriam Torres, Miguel Santin, Rogelio Martín, Fernando Alcaide
Affiliations

Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method

Raquel Moure et al. J Clin Microbiol. 2011 Mar.

Abstract

Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.

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Figures

Fig. 1.
Fig. 1.
Time to growth of Mycobacterium tuberculosis complex in liquid medium according to GX qualitative result (positive result, n = 57; negative result, n = 20). Eight samples (seven GX positive and one GX negative) were excluded due to negative growth in liquid medium (n = 4), bacterial contamination of the sample (n = 3), or lack of liquid culture (n = 1).
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