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. 2011 Jan-Feb;17(1):30-5.
doi: 10.4103/1319-3767.74461.

Non-association of IL-12 +1188 and IFN-γ +874 polymorphisms with cytokines serum level in occult HBV infected patients

Mohammad K Arababadi  1 , Ali A Pourfathollah, Abdollah Jafarzadeh, Gholamhossein Hassanshahi, Saeed Daneshmandi, Ali Shamsizadeh, Derek Kennedy
Affiliations

Non-association of IL-12 +1188 and IFN-γ +874 polymorphisms with cytokines serum level in occult HBV infected patients

Mohammad K Arababadi et al. Saudi J Gastroenterol. 2011 Jan-Feb.

Abstract

Background/aim: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-γ and +1188 of IL-12 with their serum level in patients suffering from OBI.

Materials and methods: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg-/anti-HBc+ samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-γ. In addition, the serum levels of IL-12 and IFN-γ were also determined by ELISA.

Results: Results of this study demonstrated that, 352 (9.5%) out of 3700 blood samples were HBsAg-/anti-HBc+ and HBV-DNA was detected in 57/352 (16.1%) of HBsAg-/anti-HBc+ samples. Our results showed that groups showed significant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-γ were also not associated with serum level of cytokines.

Conclusion: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-γ would not affect the expression of both cytokines at serum level in OBI patients.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Serum levels of IL-12 and IFN-γ genes in OBI and their correlation with the polymorphisms within IL-12 and IFN- patients and healthy controls. As it is obvious in figure, no signifi cant differences were seen regarding serum levels of IL-12 in OBI patients in comparison with controls (a) (P>0.1) while the serum was increased (c) (levels of IFN-γ, P<0.001). No significant differences were seen between the polymorphisms within IL-12 (b) (P>0.1) and IFN-γ (d) (P>0.1) genes and their serum levels. Data are shown as mean ± SE
See this image and copyright information in PMC

References

    1. Arababadi MK, Pourfathollah AA, Jafarzadeh A, Hassanshahi G, Rezvani ME. Association of exon 9 but not intron 8 VDR polymorphisms with occult HBV infection in south-eastern Iranian patients. J Gastroen Hepatol. 2010;25:90–3. - PubMed
    1. Arababadi MK, Hassanshahi G, Yousefi H. HBV-DNA in hemodialysis patients infected by HCV. Saudi J Kidney Dis Transpl. 2009;20:398–401. - PubMed
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    1. Jafarzadeh A, Arababadi MK, Mirzaee M, Pourazar A. Occult hepatitis B virus infection among blood donors with antibodies to hepatitis B core antigen. Acta Med Iran. 2008;46:27–32.
    1. Pourazar A, Salehi M, Jafarzadeh A, Arababadi MK, Oreizi F, Shariatinezhad K. Detection of HBV DNA in HBsAg negative normal blood donors. Iran J Immunol. 2005;2:172–6.

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