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. 2010 Nov-Dec;15(6):060503.
doi: 10.1117/1.3514633.

Spectroscopic visualization of nanoscale deformation in bone: interaction of light with partially disordered nanostructure

Zhengbin Xu  1 , Xuanhao Sun, Jingjing Liu, Qinghai Song, Matthew Muckley, Ozan Akkus, Young L Kim
Affiliations

Spectroscopic visualization of nanoscale deformation in bone: interaction of light with partially disordered nanostructure

Zhengbin Xu et al. J Biomed Opt. 2010 Nov-Dec.

Abstract

Given that bone is an intriguing nanostructured dielectric as a partially disordered complex structure, we apply an elastic light scattering-based approach to image prefailure deformation and damage of bovine cortical bone under mechanical testing. We demonstrate that our imaging method can capture nanoscale deformation in a relatively large area. The unique structure, the high anisotropic property of bone, and the system configuration further allow us to use the transfer matrix method to study possible spectroscopic manifestations of prefailure deformation. Our sensitive yet simple imaging method could potentially be used to detect nanoscale structural and mechanical alterations of hard tissue and biomaterials in a fairly large field of view.

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Figures

Figure 1
Figure 1
(a) (b) and (c) show white-light images of the bovine cortical bone specimen. (d) Typical spectra elastically backscattered from the specimen from different locations under the loading condition. The corresponding locations of blue and red spectra are marked on the white-light image in (b).
Figure 2
Figure 2
Spectroscopic images of the bovine cortical bone. Images are generated by the value of the spectral slope at each (x, y) location. A relatively large prefailure deformation zone (within the dotted line) is revealed at this small strain under tensile loading.
Figure 3
Figure 3
(a) Representative SEM image of our polished bovine bone surface. (b) Representative profile of the simulated structure. (c) Representative stimulated spectrum when the thickness of the collagen layer = 100 nm with n = 1.60 and the matrix space = 50 nm with n = 1.40.
Figure 4
Figure 4
(a) Simulation result of the 1-D multilayer dielectric nanostructure when the thickness of the mineralized collagen fibril layer was varied. (b) Simulation result when the refractive index difference was changed.
See this image and copyright information in PMC

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