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Comparative Study
. 1990 Jun 1;57(3):311-6.
doi: 10.1016/0378-1097(90)90086-6.

Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C

J Blanco  1 , M Blanco, E A González, M P Alonso, J I Garabal
Affiliations
Comparative Study

Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C

J Blanco et al. FEMS Microbiol Lett. .

Abstract

Necrotizing Escherichia coli (NTEC) strains grown in the presence of mitomycin C released cell associated necrotizing factors CNF1 and CNF2 to culture medium. Using culture filtrates from 96 mitomycin C treated E. coli strains, we have found that a modified HeLa cell assay was a more sensitive and specific method for the detection of CNF1 and CNF2 than the Vero cell assay and the rabbit skin test.

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