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Review
. 2010 Jul;1(7):621-6.
doi: 10.1007/s13238-010-0073-3. Epub 2010 Jul 29.

Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites

Hong Gao  1 , Ying Zhuo, Elizabeth Ashforth, Lixin Zhang
Affiliations
Review

Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites

Hong Gao et al. Protein Cell. 2010 Jul.

Abstract

Synthetic biology aims to design and build new biological systems with desirable properties, providing the foundation for the biosynthesis of secondary metabolites. The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology. However, there are advantages of using a deleted host, and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genome-reduced microbes. This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.

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References

    1. Agapakis C.M., Silver P.A. Synthetic biology: exploring and exploiting genetic modularity through the design of novel biological networks. Mol Biosyst. 2009;5:704–713. doi: 10.1039/b901484e. - DOI - PubMed
    1. Aho A.C., Donner K., Hydén C., Larsen L.O., Reuter T. Low retinal noise in animals with low body temperature allows high visual sensitivity. Nature. 1988;334:348–350. doi: 10.1038/334348a0. - DOI - PubMed
    1. Anné J., Van Mellaert L. Streptomyces lividans as host for heterologous protein production. FEMS Microbiol Lett. 1993;114:121–128. doi: 10.1111/j.1574-6968.1993.tb06561.x. - DOI - PubMed
    1. Bérdy J. Bioactive microbial metabolites. J Antibiot (Tokyo) 2005;58:1–26. doi: 10.1038/ja.2005.1. - DOI - PubMed
    1. Brawner M., Poste G., Rosenberg M., Westpheling J. Streptomyces: a host for heterologous gene expression. Curr Opin Biotechnol. 1991;2:674–681. doi: 10.1016/0958-1669(91)90033-2. - DOI - PubMed

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