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. 2011 Mar;50(3):578-81.
doi: 10.1016/j.yjmcc.2010.12.020. Epub 2011 Jan 6.

Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp

Tamas Banyasz  1 , Balazs Horvath, Zhong Jian, Leighton T Izu, Ye Chen-Izu
Affiliations

Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp

Tamas Banyasz et al. J Mol Cell Cardiol. 2011 Mar.

Abstract

The cardiac action potential (AP) is shaped by myriad ionic currents. In this study, we develop an innovative AP-clamp Sequential Dissection technique to enable the recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.

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Figures

Figure 1
Figure 1
A. The first current of interest, IKs is obtained by subtracting the compensation current recorded with chromanol-293B from the baseline current. B. The second current, IKr is obtained by subtracting the compensation current recorded with E4031+chromanol from the first compensation current recorded with chromanol. C&D. Representative traces of Chromanol-293B, E4031, Nisoldipine and Ba2+ sensitive currents recorded in guinea pig ventricular myocytes. The sequence of channel blocker represents the order of drug application. E. Correlation between QNISO and QKs. F. Correlation between the inward going charges carried by INISO and the sum of outward going charges carried by IKs, IKr, IK1.
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