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. 2011 Feb;8(2):143-6.
doi: 10.1038/nmeth.1553. Epub 2011 Jan 9.

A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy

Luis A Campos  1 , Jianwei Liu, Xiang Wang, Ravishankar Ramanathan, Douglas S English, Victor Muñoz
Affiliations

A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy

Luis A Campos et al. Nat Methods. 2011 Feb.

Abstract

Time resolution of current single-molecule fluorescence techniques is limited to milliseconds because of dye blinking and bleaching. Here we introduce a photoprotection strategy that affords microsecond resolution by combining efficient triplet quenching by oxygen and Trolox with minimized bleaching via the oxygen radical scavenger cysteamine. Using this approach we resolved the single-molecule microsecond conformational fluctuations of two proteins: the two-state folder α-spectrin SH3 domain and the ultrafast downhill folder BBL.

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References

    1. Curr Opin Struct Biol. 2008 Feb;18(1):16-26 - PubMed
    1. J Phys Chem B. 2005 Sep 22;109(37):17683-8 - PubMed
    1. Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12596-602 - PubMed
    1. Free Radic Biol Med. 1999 Apr;26(7-8):961-7 - PubMed
    1. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1379-84 - PubMed

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