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. 1990 Oct;28(10):2200-4.
doi: 10.1128/jcm.28.10.2200-2204.1990.

Polymerase chain reaction for detection of Mycobacterium tuberculosis

U Sjöbring  1 , M Mecklenburg, A B Andersen, H Miörner
Affiliations

Polymerase chain reaction for detection of Mycobacterium tuberculosis

U Sjöbring et al. J Clin Microbiol. 1990 Oct.

Abstract

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

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