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. 2010 Mar;3(1):35-9.
doi: 10.2478/v10102-010-0002-6. Epub 2010 Mar 29.

Investigation of in vitro effects of ethephon and chlorpyrifos, either alone or in combination, on rat intestinal muscle contraction

Mustafa Alp Cetinkaya  1 , Emine Baydan
Affiliations

Investigation of in vitro effects of ethephon and chlorpyrifos, either alone or in combination, on rat intestinal muscle contraction

Mustafa Alp Cetinkaya et al. Interdiscip Toxicol. 2010 Mar.

Abstract

A range of pesticides is widely used in pest management and the chances of exposure to multiple organophosphorus (OP) compounds simultaneously are high, especially from dietary and other sources. Although health hazards of individual OP insecticides have been relatively well characterized, there is lesser information on the interactive toxicity of multiple OP insecticides. The aim of this study is to elicit the possible interactions in case combined exposure of an OP pesticide chlorpyrifos (CPF) and a plant growth regulator ethephon (ETF) which are used worldwide. The ileum segments of 3 months old Wistar Albino male rats were used in isolated organ bath containing Tyrode solution. ETF and CPF were incubated (10(-7) M concentration) separately or in combination with each other to ileum and their effects on acetylcholine-induced contractions were studied. The data obtained from this study show that, single and combined exposure to the agents caused agonistic interactions with regard to potency of ACh whereas they caused a decrease on E(max) value of ACh. These findings suggest that exposure to these agents which have direct and indirect cholinergic effects, may cause developing clinical responses with small doses and earlier but the extent of toxicity will be lower.

Keywords: acetylcholine; chlorpyrifos; combination; ethephon; organophosphate.

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Figures

Figure 1
Figure 1
The average mg values of the highest contractions and the responses of cumulatively applied ETF on ileum.
Figure 2
Figure 2
The average mg values of the highest contractions and the responses of cumulatively applied CPF on ileum.
Figure 3
Figure 3
Cumulative concentration-response of ACh that is appropriate to specific graded concentration-response.
See this image and copyright information in PMC

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