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. 2011 Apr;55(4):1792-3.
doi: 10.1128/AAC.01403-10. Epub 2011 Jan 10.

Restoration of chloride efflux by azithromycin in airway epithelial cells of cystic fibrosis patients

Vinciane Saint-Criq  1 , Carine Rebeyrol, Manon Ruffin, Telma Roque, Loïc Guillot, Jacky Jacquot, Annick Clement, Olivier Tabary
Affiliations

Restoration of chloride efflux by azithromycin in airway epithelial cells of cystic fibrosis patients

Vinciane Saint-Criq et al. Antimicrob Agents Chemother. 2011 Apr.

Abstract

Azithromycin (AZM) has shown promising anti-inflammatory properties in chronic obstructive pulmonary diseases, and clinical studies have presented an improvement in the respiratory condition of cystic fibrosis (CF) patients. The aim of this study was to investigate, in human airway cells, the mechanism by which AZM has beneficial effects in CF. We demonstrated that AZM did not have any anti-inflammatory effect on CF airway cells but restored Cl(-) efflux.

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Figures

FIG. 1.
FIG. 1.
Effect of AZM (10 μg/ml) on chloride efflux in CF airway epithelial cells (IB3-1, CFBE41o−, and CF hAEC) and in non-CF airway cells (S9 cells). (A) Cells were loaded with SPQ probe, and fluorescence was measured every second after treatment with AZM. Data are relative fluorescence Ft/F0, where “Ft” is the fluorescence intensity at time T and “F0” is the fluorescence at time zero. (B) The histogram represents the means of activation at 600 s of 35 cells in 5 independent experiments. ***, P < 0.001.
See this image and copyright information in PMC

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