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. 2011:715:81-92.
doi: 10.1007/978-1-61779-008-9_5.

Carbohydrate gel electrophoresis

Florence Goubet  1 , Paul Dupree, Katja Salomon Johansen
Affiliations

Carbohydrate gel electrophoresis

Florence Goubet et al. Methods Mol Biol. 2011.

Abstract

Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.

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