Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar;49(3):1104-6.
doi: 10.1128/JCM.01777-10. Epub 2011 Jan 12.

Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Marc Roger Couturier  1 , Elvina Mehinovic, Ann C Croft, Mark A Fisher
Affiliations

Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Marc Roger Couturier et al. J Clin Microbiol. 2011 Mar.

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and accurate tool for the identification of many microorganisms. We assessed this technology for the identification of 103 Haemophilus parainfluenzae, Aggregatibacter aphrophilus, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae (HACEK) clinical isolates and 20 Haemophilus influenzae clinical isolates. Ninety-three percent of HACEK organisms were identified correctly to the genus level using the Bruker database, and 100% were identified to the genus level using a custom database that included clinical isolates.

PubMed Disclaimer

Figures

Fig. 1.
Fig. 1.
Organism-specific breakdown of identification scores achieved for HACEK organisms and H. influenzae using the standard Biotyper database. Bars represent the percentages of the total numbers of isolates for each species.
Fig. 2.
Fig. 2.
Score comparison of C. hominis (A) and A. aphrophilus (B) identified using the Bruker database and a custom database that included representative clinical isolates for each species.
See this image and copyright information in PMC

References

    1. Bessede E., et al. 27 May 2010, posting date Matrix-assisted laser-desorption/ionization biotyper: experience in the routine of a University hospital. Clin. Microbiol. Infect. doi:10.1111/j.1469-0691.2010.03274.x - DOI - PubMed
    1. Bizzini A., Durussel C., Bille J., Greub G., Prod'hom G. 2010. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J. Clin. Microbiol. 48:1549–1554 - PMC - PubMed
    1. Ferrieri P., et al. 2002. Unique features of infective endocarditis in childhood. Pediatrics 109:931–943 - PubMed
    1. Fournier P. E., Couderc C., Buffet S., Flaudrops C., Raoult D. 2009. Rapid and cost-effective identification of Bartonella species using mass spectrometry. J. Med. Microbiol. 58:1154–1159 - PubMed
    1. Haag A. M., Taylor S. N., Johnston K. H., Cole R. B. 1998. Rapid identification and speciation of Haemophilus bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Mass Spectrom. 33:750–756 - PubMed

Publication types

MeSH terms

LinkOut - more resources