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. 2011 Apr;190(1):57-74.
doi: 10.1111/j.1469-8137.2010.03601.x. Epub 2011 Jan 13.

AtCPL5, a novel Ser-2-specific RNA polymerase II C-terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis

Yong-Mei Jin  1 , Jinwook Jung  1 , Hyesung Jeon  1 , So Youn Won  1   2 , Yue Feng  3 , Jae-Sook Kang  4 , Sang Yeol Lee  4 , Jong-Joo Cheong  1 , Hisashi Koiwa  3 , Minkyun Kim  1
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Free article

AtCPL5, a novel Ser-2-specific RNA polymerase II C-terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis

Yong-Mei Jin et al. New Phytol. 2011 Apr.
Free article

Abstract

Arabidopsis RNA polymerase II (RNAPII) C-terminal domain (CTD) phosphatases regulate stress-responsive gene expression and plant development via the dephosphorylation of serine (Ser) residues of the CTD. Some of these phosphatases (CTD phosphatase-like 1 (CPL1) to CPL3) negatively regulate ABA and stress responses. Here, we isolated AtCPL5, a cDNA encoding a protein containing two CTD phosphatase domains (CPDs). To characterize AtCPL5, we analyzed the gene expression patterns and subcellular protein localization, investigated various phenotypes of AtCPL5-overexpressors and knockout mutants involved in ABA and drought responses, performed microarray and RNA hybridization analyses using AtCPL5-overexpressors, and assessed the CTD phosphatase activities of the purified AtCPL5 and each CPD of the protein. Transcripts of the nucleus-localized AtCPL5 were induced by ABA and drought. AtCPL5-overexpressors exhibited ABA-hypersensitive phenotypes (increased inhibition of seed germination, seedling growth, and stomatal aperture), lower transpiration rates upon dehydration, and enhanced drought tolerance, while the knockout mutants showed weak ABA hyposensitivity. AtCPL5 overexpression changed the expression of numerous genes, including those involved in ABA-mediated responses. In contrast to Ser-5-specific phosphatase activity of the negative stress response regulators, purified AtCPL5 and each CPD of the protein specifically dephosphorylated Ser-2 in RNAPII CTD. We conclude that AtCPL5 is a unique CPL family protein that positively regulates ABA-mediated development and drought responses in Arabidopsis.

Keywords: ABA; Arabidopsis; CPL; RNAPII CTD phosphatase; drought stress.

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References

    1. Asselbergh B, De Vleesschauwer D, Höfte M. 2008. Global switches and fine-tuning-ABA modulates plant pathogen defense. Molecular Plant-Microbe Interactions 21: 709-719.
    1. Bang W, Kim S, Ueda A, Vikram M, Yun D, Bressan RA, Hasegawa PM, Bahk J, Koiwa H. 2006. Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions. Plant Physiology 142: 586-594.
    1. Bang WY, Kim SW, Jeong IS, Koiwa H, Bahk JD. 2008. The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. Biochemical and Biophysical Research Communications 372: 907-912.
    1. Bohlmann H, Vignutelli A, Hilpert B, Miersch O, Wasternack C, Apel K. 1998. Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway. FEBS Letters 437: 281-286.
    1. Bolstad BM, Irizarry RA, Astrand M, Speed TP. 2003. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19: 185-193.

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