doi: 10.1039/c0mb00217h.
Epub 2011 Jan 14.
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives
Affiliations
- PMID: 21234492
- DOI: 10.1039/c0mb00217h
Item in Clipboard
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives
Mol Biosyst.
2011 Mar.
Abstract
Using evolved pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs, L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.
Similar articles
-
A genetically encoded photocaged Nepsilon-methyl-L-lysine.Mol Biosyst. 2010 Sep;6(9):1557-60. doi: 10.1039/c002155e. Epub 2010 Mar 30. Mol Biosyst. 2010. PMID: 20711534
-
Recognition of non-alpha-amino substrates by pyrrolysyl-tRNA synthetase.J Mol Biol. 2009 Feb 6;385(5):1352-60. doi: 10.1016/j.jmb.2008.11.059. Epub 2008 Dec 11. J Mol Biol. 2009. PMID: 19100747
-
High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites.Biochem Biophys Res Commun. 2012 Feb 24;418(4):652-6. doi: 10.1016/j.bbrc.2012.01.069. Epub 2012 Jan 24. Biochem Biophys Res Commun. 2012. PMID: 22293204
-
[Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality].Tanpakushitsu Kakusan Koso. 2009 Sep;54(12 Suppl):1660-9. Tanpakushitsu Kakusan Koso. 2009. PMID: 21089604 Review. Japanese. No abstract available.
-
tRNAPyl: Structure, function, and applications.RNA Biol. 2018;15(4-5):441-452. doi: 10.1080/15476286.2017.1356561. Epub 2017 Sep 13. RNA Biol. 2018. PMID: 28837402 Free PMC article. Review.
Cited by
-
Multiple site-selective insertions of noncanonical amino acids into sequence-repetitive polypeptides.Chembiochem. 2013 May 27;14(8):968-78. doi: 10.1002/cbic.201300069. Epub 2013 Apr 26. Chembiochem. 2013. PMID: 23625817 Free PMC article.
-
Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli.ACS Synth Biol. 2016 Feb 19;5(2):163-71. doi: 10.1021/acssynbio.5b00197. Epub 2015 Nov 20. ACS Synth Biol. 2016. PMID: 26544153 Free PMC article.
-
Monitoring monomer-specific acyl-tRNA levels in cells with PARTI.Nucleic Acids Res. 2025 Apr 22;53(8):gkaf327. doi: 10.1093/nar/gkaf327. Nucleic Acids Res. 2025. PMID: 40335069 Free PMC article.
-
Noncanonical Amino Acid Tools and Their Application to Membrane Protein Studies.Chem Rev. 2024 Nov 27;124(22):12498-12550. doi: 10.1021/acs.chemrev.4c00181. Epub 2024 Nov 7. Chem Rev. 2024. PMID: 39509680 Free PMC article. Review.
-
The Impact of Hidden Structure on Aggregate Disassembly by Molecular Chaperones.Front Mol Biosci. 2022 Jul 7;9:915307. doi: 10.3389/fmolb.2022.915307. eCollection 2022. Front Mol Biosci. 2022. PMID: 35874607 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
