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Comparative Study
. 2011 Mar;5(2):99-103.
doi: 10.1111/j.1750-2659.2010.00170.x.

Comparison of a singleplex real-time RT-PCR assay and multiplex respiratory viral panel assay for detection of influenza "A" in respiratory specimens

Kanti Pabbaraju  1 , Sallene Wong, Bonita Lee, Raymond Tellier, Kevin Fonseca, Marie Louie, Steven J Drews
Affiliations
Comparative Study

Comparison of a singleplex real-time RT-PCR assay and multiplex respiratory viral panel assay for detection of influenza "A" in respiratory specimens

Kanti Pabbaraju et al. Influenza Other Respir Viruses. 2011 Mar.

Abstract

Background: Evaluation of different molecular tests for the detection of pandemic (H1N1) 2009 virus is important before the next wave of the pandemic.

Objectives: To compare a hydrolysis probe-based real-time RTPCR assay recommended by the CDC to the xTAG respiratory viral panel (RVP) (Luminex Molecular Diagnostics) for the detection of influenza A.

Methods: Eleven thousand eight hundred and ninety-eight respiratory specimens were tested by the real-time RT-PCR and RVP assays for the detection of influenza A. The distribution of seasonal H1, H3 and pandemic H1N1 subtypes in these specimens was compared.

Results: The RVP assay was generally unable to identify influenza A–positive samples with a low viral load, whereas the real-time RT-PCR assay detected most of these samples resulting in a subset of specimens that could not be confirmed as either seasonal or pandemic influenza A subtypes.

Conclusions: When the prevalence of influenza A is high, the CDC recommended real-time RT-PCR has significant advantages as a frontline assay, namely higher sensitivity and shorter time to reporting a result. Anticipated scenarios would be during the peaks of the pandemic and episodes of seasonal influenza. Furthermore, the better sensitivity of the RT-PCR makes it the preferred assay to detect influenza in patients with severe respiratory disease tested late in their clinical course. If pandemic (H1N1) 2009 virus is not the dominant virus and there is a high proportion of other respiratory viruses circulating, laboratories will be faced with the decision to use the RVP assay for the detection of pandemic (H1N1) 2009 virus.

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Figures

Figure 1
Figure 1
Typing of samples positive for influenza A by respiratory viral panel (RVP) and CDC‐M assays. The pie chart shows a distribution of influenza A subtypes as detected by the RVP assay, subtyping of samples that could not be typed by the RVP assay is provided by the bar graph. *One sample was not typed; another sample from the same patient was typed as pandemic (H1N1) 2009 virus. One sample was subtyped by sero‐typing.
See this image and copyright information in PMC

References

    1. Carr MJ, Gunson R, Maclean A et al. Development of a real‐time RT‐PCR for the detection of swine‐lineage influenza A (H1N1) virus infections. J Clin Virol 2009; 45:196–199. - PMC - PubMed
    1. Fouchier RA, Bestebroer TM, Herfst S, Van Der KL, Rimmelzwaan GF, Osterhaus AD. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. J Clin Microbiol 2000; 38:4096–4101. - PMC - PubMed
    1. Ginocchio CC, Zhang F, Manji R et al. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. J Clin Virol 2009; 45:191–195. - PMC - PubMed
    1. Liu S, Hou G, Zhuang Q et al. A SYBR Green I real‐time RT‐PCR assay for detection and differentiation of influenza A(H1N1) virus in swine populations. J Virol Methods 2009; 162:184–187. - PubMed
    1. Panning M, Eickmann M, Landt O et al. Detection of influenza A(H1N1)v virus by real‐time RT‐PCR. Euro Surveill 2009; 14:19329–19334. - PubMed

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