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Comparative Study
. 2011 Mar;49(3):1124-7.
doi: 10.1128/JCM.02104-10. Epub 2011 Jan 19.

Colorimetric-plate method for rapid disk diffusion susceptibility testing of Escherichia coli

Sehnaz Sener  1 , Ibrahim Cagatay Acuner, Yuksel Bek, Belma Durupinar
Affiliations
Comparative Study

Colorimetric-plate method for rapid disk diffusion susceptibility testing of Escherichia coli

Sehnaz Sener et al. J Clin Microbiol. 2011 Mar.

Abstract

Here, we report a laboratory-developed colorimetric-plate method for rapid disk diffusion susceptibility testing of Escherichia coli. One hundred isolates were evaluated. Categorical agreement between the colorimetric plate and the standard disk diffusion method was 99%. Mean time to results was 7.07 h (95% confidence interval, 5.96 to 8.19).

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Figures

Fig. 1.
Fig. 1.
View of the in-house colorimetric plate for susceptibility testing of E. coli after an incubation period of 7 h. Due to the redox indicator resazurin, a background color change from blue (resazurin) to pink (resorufin) is observed in the zones of the plate where E. coli colonies grow.
See this image and copyright information in PMC

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