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. 2011 Jan 21;17(3):329-33.
doi: 10.3748/wjg.v17.i3.329.

Low red blood cell levels of deglycating enzymes in colorectal cancer patients

Maria Notarnicola  1 , Maria Gabriella Caruso, Valeria Tutino, Vito Guerra, Giovanni Misciagna
Affiliations

Low red blood cell levels of deglycating enzymes in colorectal cancer patients

Maria Notarnicola et al. World J Gastroenterol. .

Abstract

Aim: To investigate Glyoxalase I and fructosamine-3-kinase (FN3K) activity in red blood cells from patients with colorectal adenomas and cancer.

Methods: Thirty three consecutive subjects with one or more histologically confirmed colorectal adenomatous polyps, 16 colorectal cancer patients and a group of 11 control subjects with normal colonoscopy were included in the study. Glyoxalase I and FN3K activities were measured in red blood cells using a spectrophotometric and radiometric assay, respectively.

Results: A significant reduction in both Glyoxalase I and FN3K activity was detected in patients with tumors compared to patients with adenomas and the controls. Erythrocyte Glyoxalase I activity in colorectal cancer was approximately 6 times lower than that detected in patients with adenoma (0.022 ± 0.01 mmol/min per milliliter vs 0.128 ± 0.19 mmol/min per milliliter of red blood cells, P = 0.003, Tukey's test). FN3K activity in red blood cells from patients with colon cancer was approximately 2 times lower than that detected in adenoma patients (19.55 ± 6.4 pmol/min per milliliter vs 38.6 ± 31.7 pmol/min per milliliter of red blood cells, P = 0.04, Tukey's test).

Conclusion: These findings suggest that deglycating enzymes may be involved in the malignant transformation of colon mucosa.

Keywords: Colorectal cancer; Enzymatic activity; Fructosamine-3-kinase; Glycation; Glyoxalase I.

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