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. 2010:2010:250606.
doi: 10.1155/2010/250606. Epub 2010 Dec 28.

Immunohistochemical Expression of p53 in Pleomorphic Adenoma and Carcinoma Ex Pleomorphic Adenoma

Bassel Tarakji  1 , Omar Kujan, Mohammad Z Nassani
Affiliations

Immunohistochemical Expression of p53 in Pleomorphic Adenoma and Carcinoma Ex Pleomorphic Adenoma

Bassel Tarakji et al. J Cancer Epidemiol. 2010.

Abstract

Context. Immunohistochemical stains for p53 are used as a diagnostic marker associated with malignancy in several histologic types of salivary gland tumors. This marker may be useful in differentiating pleomorphic adenoma (PA) from carcinoma ex pleomorphic adenoma (CPA), as these tumors are often difficult to distinguish on the basis of morphology alone. Objective. to evaluate whatever inactivation of tumor suppressor gene (p53) increases with the tumor progression from normal salivary tissue to PA and eventually CPA. Design. Paraffin blocks of 29 cases of PA, which were surrounded by normal parotid gland, and 27 cases of carcinoma ex pleomorphic adenoma were retrieved and validated. In all cases of carcinoma ex pleomorphic adenoma, a PA "ghost" was identified, and the malignant element was either undifferentiated carcinoma or adenocarcinoma. Results. The results showed negative nuclear expression of P53 in normal parotid gland. Nuclear P53 was expressed strongly in 6/29 (20.7%) pleomorphic salivary adenoma and 10/27 (37%) carcinoma ex pleomorphic adenoma. Conclusion. Our data suggest that inactivation of p53 may play an important role in the evolution of pleomorphic salivary adenoma and carcinoma ex pleomorphic adenoma.

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Figures

Figure 1
Figure 1
Showing negative nuclear staining of p53 in normal salivary tissue. Original magnification x40.
Figure 2
Figure 2
Showing strong p53 staining in pleomorphic salivary adenoma. Original magnification x40.
Figure 3
Figure 3
Showing moderate p53 staining in pleomorphic salivary adenoma. Original magnification x40.
Figure 4
Figure 4
Showing low nuclear staining of p53 in pleomorphic salivary adenoma. Original magnification x40.
Figure 5
Figure 5
Showing strong p53 staining of nuclei in CPA. Original magnification x40.
Figure 6
Figure 6
Showing moderate p53 nuclear staining in CPA. Original magnification x40.
Figure 7
Figure 7
Showing low nuclear staining of p53 in CPA. Original magnification x40.
Figure 8
Figure 8
Showing p53 negative control for CPA. Original magnification x40.
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