Detection of Listeria monocytogenes by using the polymerase chain reaction
- PMID: 2125817
- PMCID: PMC184869
- DOI: 10.1128/aem.56.9.2930-2932.1990
Detection of Listeria monocytogenes by using the polymerase chain reaction
Abstract
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
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