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Comment
. 2011 Feb;10(2):83-4.
doi: 10.1038/nmat2955.

Protein polymer: Gene libraries open up

Sheng Ding, Xiaoxiao Wang, Annelise E Barron
Comment

Protein polymer: Gene libraries open up

Sheng Ding et al. Nat Mater. 2011 Feb.
No abstract available

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Figures

Figure 1
Figure 1
A schematic of strategies to synthesize oligomer genes. a, Concatemerization. The double-stranded monomer genes are generated either by enzyme digestion from original plasmid or by annealing of two complementary single-stranded DNA fragments. Then, monomer fragments are ligated to form oligomers of different lengths. Type IIS restriction endonuclease can be used to eliminate sequence constraints and introduce direction control in oligomization. b, Overlap-extension PCR (OEPCR). Two ssDNA fragments anneal to each other through their overlapping regions, and are extended to longer sizes during chain extension steps. c, Rolling circle amplification (RCA). Cyclized DNA is used as the template in RCA. During the elongation cycle the polymerase rolls over the template and extends continuously to yield linear complementary single-stranded DNA fragments of various lengths.
See this image and copyright information in PMC

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