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Comparative Study
. 1990 Dec;81(12):1253-8.
doi: 10.1111/j.1349-7006.1990.tb02687.x.

Species difference among experimental rodents in the activity and induction of cytochrome P-450 isozymes for mutagenic activation of carcinogenic aromatic amines

M Degawa  1 , T Agatsuma, Y Hashimoto
Affiliations
Comparative Study

Species difference among experimental rodents in the activity and induction of cytochrome P-450 isozymes for mutagenic activation of carcinogenic aromatic amines

M Degawa et al. Jpn J Cancer Res. 1990 Dec.

Abstract

The expressions of hepatic microsomal cytochrome P-450 isozymes in male rats, mice, hamsters and guinea pigs were studied comparatively with or without an ip injection of a cytochrome P-450 inducer. The activity and quantity of microsomal cytochrome P-450 isozymes were determined respectively by a bacterial mutation assay with Salmonella typhimurium TA98 and immunochemical assays using monoclonal antibodies against rat cytochrome P-450 isozymes. 3-Methoxy-4-aminoazobenzene (3-MeO-AAB), 2-amino-3-methyl-9H-pyrido[2,3-b]indole acetate (MeA alpha C) and 3-methylcholanthrene were used as cytochrome P-450 inducers, and 7 carcinogenic aromatic amines including 3-MeO-AAB and MeA alpha C were used as substrates for the mutation assay. By means of these assays, we examined the species differences among rodents in the activity and induction rate of hepatic cytochrome P-450 isozymes responsible for the mutagenic activation of carcinogenic aromatic amines.

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References

    1. ) Degawa , M. , Kojima , M. and Hashimoto , Y.Species difference between rats and mice in activities of enzymes activating aromatic amines: effect of dietary 3‐methoxy‐4‐aminoazobenzene . Gann , 75 , 966 – 975 ( 1984. ). - PubMed
    1. ) Degawa , M. , Kojima , M. , Masuko , T. , Hishinuma , T. and Hashimoto , Y.3‐Methoxy‐4‐aminoazobenzene, a selective inducer for a high spin form of cytochrome P‐448 in rat liver microsomes . Biochem. Biophys. Res. Commun. , 133 , 1072 – 1077 ( 1985. ). - PubMed
    1. ) Degawa , M. , Kojima , M. , Sato , Y. and Hashimoto , Y.Induction of a high spin form of microsomal cytochrome P‐448 in rat liver by 4‐aminoazobenzene derivatives . Biochem. Pharmacol. , 35 , 3565 – 3570 ( 1986. ). - PubMed
    1. ) Degawa , M. , Yamada , H. , Hishinuma , T. and Hashimoto , Y.Organ selective induction of cytochrome P‐448 isozymes in rat by 2‐methoxy‐4‐aminoazobenzene and 3‐methylcholanthrene . J. Biochem. , 101 , 1417 – 1445 ( 1987. ). - PubMed
    1. ) Hashimoto , Y. , Degawa , M. , Kojima , M. and Hishinuma , T.Induction of carcinogen activation enzyme(s) by feeding of a carcinogenic tryptophan pyrolysate correlates to sex difference in the carcinogenesis of the mouse . Gann , 73 , 508 – 510 ( 1982. ). - PubMed

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