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. 2011 Jan 15;36(2):139-41.
doi: 10.1364/OL.36.000139.

Fast voice-coil scanning optical-resolution photoacoustic microscopy

Lidai Wang  1 , Konstantin Maslov, Junjie Yao, Bin Rao, Lihong V Wang
Affiliations

Fast voice-coil scanning optical-resolution photoacoustic microscopy

Lidai Wang et al. Opt Lett. .

Abstract

We developed a photoacoustic imaging system that has real-time imaging capability with optical resolution. The imaging system is capable of scanning at 20 Hz over a 9 mm range and up to 40 Hz over a 1 mm scanning range. A focused laser beam provides a lateral resolution of 3.4 μm as measured in an optically nonscattering medium. Flows of micrometer-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, demonstrating the capability to image highly dynamic processes in vivo at a micrometer-scale resolution.

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Figures

Fig. 1
Fig. 1
Schematic of voice-coil-driven fast-scanning OR-PAM.
Fig. 2
Fig. 2
(A) Lateral resolution test on a sharp edge. ESF, edge spread function; LSF, line spread function. (B) Test of penetration depth by imaging a needle obliquely inserted into biological tissue. (C) In vivo maximum amplitude projection (MAP) image of mouse ear vasculature. (D) Close-up of the region enclosed by the dashed box in (C); arrows denote capillaries. NPA, normalized photoacoustic amplitude.
Fig. 3
Fig. 3
(A) Schematic of the experiment setup for carbon particle flow measurement. (B) Representative B-scan flow image across the dashed-box area in Fig. 3(A). (C) Distribution of imaged carbon particles along the x-axis at one z-axis position versus time. (D) Frequency spectrum of data in Fig. 3(C) obtained with two-dimensional Fourier transformation. (E) Imaged parabolic flow speed along the z-axis. NPA, normalized photoacoustic amplitude; NSD, normalized spectrum density.
Fig. 4
Fig. 4
(A) B-scan image of whole blood flowing in a tube. (B) MAP along the z-axis of the B-scan image versus time. (C) Two-dimensional frequency spectrum of the data in Fig. 4(B). (D) Measured flow speed versus the preset values. NPA, normalized photoacoustic amplitude; NSD, normalized spectrum density.
Fig. 5
Fig. 5
(A) In vivo B-scan of single RBCs flowing in a mouse ear capillary. (B) MAP along the z-axis of the B-scan image versus time (Media 1). (C) Two-dimensional frequency spectrum of the data in Fig. 5(B). NPA: normalized photoacoustic amplitude; NSD: normalized spectrum density.
See this image and copyright information in PMC

References

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