Increased bioactivity of serum follicle-stimulating hormone, but not luteinizing hormone, following hemicastration in ram lambs

Abstract

This study evaluated the short-term effects of hemicastration on levels of bioactive (bio-) and immunoactive (immuno-) FSH and LH in 3.5-mo-old ram lambs. Biological activities of FSH and LH were determined using in vitro rat granulosa cell aromatase and mouse Leydig cell testosterone assays, respectively. Four rams each were either hemicastrated (HC) or left intact (INT) and blood samples were collected over a 2-wk period. In HC rams, serum bio- and immuno-FSH concentrations increased several fold within 5-8 h after surgery and remained elevated over INT controls for 7 and 14 days, respectively. The increase in bio-FSH was greater than that for immuno-FSH, resulting in an increase in the ratio of biological to immunological activity (B/I ratio) through Day 7. Neither immuno-FSH, bio-FSH, nor the B/I ratios varied throughout the 2-wk study in INT rams. In contrast to FSH, serum concentrations of immuno- and bio-LH and the B/I ratios were similar between HC and INT rams. For both gonadotropins, biological activity was positively correlated to immunological activity. These results demonstrate that (1) immuno- and bio-FSH concentrations are elevated after hemicastration; (2) the FSH biopotency (B/I ratio) also is increased, at least temporarily; and (3), despite these changes in FSH production, neither immuno- nor bio-LH secretion is altered by hemicastration.