Novel nested direct PCR technique for malaria diagnosis using filter paper samples
- PMID: 21270224
- PMCID: PMC3122810
- DOI: 10.1128/JCM.01792-10
Novel nested direct PCR technique for malaria diagnosis using filter paper samples
Abstract
The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.
References
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- Chaorattanakawee S., et al. 2003. Storage duration and polymerase chain reaction detection of Plasmodium falciparum from blood spots on filter paper. Am. J. Trop. Med. Hyg. 69:42–44 - PubMed
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- Finnzymes 2008. Phusion Blood direct PCR kit: instruction manual. Finnzymes Oy, Espoo, Finland
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- Noedl H., Socheat D., Satimai W. 2009. Artemisinin-resistant malaria in Asia. N. Engl. J. Med. 361:540–541 - PubMed
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