Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Apr;49(4):1628-30.
doi: 10.1128/JCM.01792-10. Epub 2011 Jan 26.

Novel nested direct PCR technique for malaria diagnosis using filter paper samples

Affiliations

Novel nested direct PCR technique for malaria diagnosis using filter paper samples

Hans-Peter Fuehrer et al. J Clin Microbiol. 2011 Apr.

Abstract

The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.

PubMed Disclaimer

References

    1. Chaorattanakawee S., et al. 2003. Storage duration and polymerase chain reaction detection of Plasmodium falciparum from blood spots on filter paper. Am. J. Trop. Med. Hyg. 69:42–44 - PubMed
    1. Finnzymes 2008. Phusion Blood direct PCR kit: instruction manual. Finnzymes Oy, Espoo, Finland
    1. Fuehrer H. P., et al. 2010. Indigenous Plasmodium ovale malaria in Bangladesh. Am. J. Trop. Med. Hyg. 83:75–78 - PMC - PubMed
    1. Imwong M., et al. 2009. Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. J. Clin. Microbiol. 47:4173–4175 - PMC - PubMed
    1. Noedl H., Socheat D., Satimai W. 2009. Artemisinin-resistant malaria in Asia. N. Engl. J. Med. 361:540–541 - PubMed

Publication types

MeSH terms