Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2011 Apr;49(4):1202-5.
doi: 10.1128/JCM.02268-10. Epub 2011 Jan 26.

Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system

Doris Hillemann  1 , Sabine Rüsch-Gerdes, Catharina Boehme, Elvira Richter
Affiliations
Comparative Study

Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system

Doris Hillemann et al. J Clin Microbiol. 2011 Apr.

Abstract

In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-based GeneXpert MTB/RIF (Xpert) assay system and conventional liquid and solid culture methods. Twenty (3.8%) of the 521 specimens gave no interpretable result. Whereas the sensitivity of the Xpert assay with tissue specimens was 69.0% (20 out of 29 culture-positive cases detected), 100% sensitivity was found with the urine and stool specimens. The combined sensitivity and specificity of the Xpert assay were calculated to be 77.3% and 98.2%, respectively.

PubMed Disclaimer

References

    1. Reference deleted.
    1. Boehme C. C., et al. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005–1015 - PMC - PubMed
    1. Cheng V. C., et al. 2004. Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis. J. Clin. Pathol. 57:281–285 - PMC - PubMed
    1. Deutsches Institut für Normung 1986. Medical microbiology. Diagnosis of tuberculosis. Part 3. Detection of mycobacteria by culture methods. DIN 58943-3. Beuth Verlag, Berlin, Germany
    1. Deutsches Institut für Normung 1995. Medizinische Mikrobiologie. Tuberkulosediagnostik. Teil 32. Mikroskopische Methoden zum Nachweis von Mykobakterien. DIN 58943-32. Beuth Verlag, Berlin, Germany

MeSH terms