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. 2011 Apr;49(4):1653-6.
doi: 10.1128/JCM.02184-10. Epub 2011 Jan 26.

Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR

Yu Chen  1 , Dawei Cui, Shufa Zheng, Shigui Yang, Jia Tong, Dagan Yang, Jian Fan, Jie Zhang, Bin Lou, Xuefen Li, Xiaoling Zhuge, Bo Ye, Baode Chen, Weilin Mao, Yajun Tan, Genyun Xu, Zhenjin Chen, Nan Chen, Lanjuan Li
Affiliations

Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR

Yu Chen et al. J Clin Microbiol. 2011 Apr.

Abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

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References

    1. Beck E. T., et al. 2010. Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. J. Mol. Diagn. 12:74–81 - PMC - PubMed
    1. Briese T., et al. 2005. Diagnostic system for rapid and sensitive differential detection of pathogens. Emerg. Infect. Dis. 11:310–313 - PMC - PubMed
    1. Centers for Disease Control and Prevention 2009. Swine influenza A (H1N1) infection in two children—southern California, March-April 2009. MMWR Morb. Mortal. Wkly. Rep. 58:400–402 - PubMed
    1. Coiras M. T., Aguilar J. C., Garcia M. L., Casas I., Perez-Brena P. 2004. Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays. J. Med. Virol. 72:484–495 - PMC - PubMed
    1. Dong H., et al. 2010. Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR. Virus. Res. 147:85–90 - PubMed

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