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. 2011 Feb;27(1):15-22.
doi: 10.1007/s12264-011-1042-4.

Expressions of Axl and Tyro-3 receptors are under regulation of nerve growth factor and are involved in differentiation of PC12 cells

Qi Wang  1 , Qing-Jun Lu, Bing Xiao, Yan Zheng, Xiao-Min Wang
Affiliations

Expressions of Axl and Tyro-3 receptors are under regulation of nerve growth factor and are involved in differentiation of PC12 cells

Qi Wang et al. Neurosci Bull. 2011 Feb.

Abstract
in English, Chinese

Objective: Tyro-3 and Axl receptors are expressed in brain in a region-specific manner and their bioactivities in the central nervous system remain still elusive. The aim of the present study was to investigate their functions in neuronal differentiation.

Methods: PC12 cells overexpressing Tyro-3 or Axl were established by transfection with full-length CMV-Tyro-3-eCFP or CMV-Axl-eGFP plasmid, respectively. CMV-eGFP plasmid served as a control vector. After that, the fluorescence intensity and distributions of green fluorescent protein (GFP) and cyan fluorescent protein (CFP) in the cells with or without nerve growth factor (NGF) treatment were real-time monitored.

Results: Expressions of Tyro-3 and Axl receptors were under the regulation of NGF and associated with neuronal differentiation. This was not observed in CMV-eGFP-transfected PC12 cells. Besides, confocal microscopy revealed that NGF affected intracellular localization of full-length Axl-eGFP and Tyro-3-eCFP in PC12 cells. Moreover, the development of outgrowth of differentiated PC12 cells under stimulation of NGF was promoted by overexpression of Tyro-3 or Axl.

Conclusion: Expressions of Tyro-3 and Axl receptors are under the regulation of NGF and are involved in NGF-induced neuronal differentiation of PC12 cells.

目的: Axl 和Tyro-3 受体在脑内有区域性的分布, 但两者在中枢神经系统中的生物学功能尚不明确。 本研究旨在探讨Axl和Tyro-3受体在神经元分化中的作用。

方法: PC12细胞分别转染CMV-Axl-eGFP、 CMV-Tyro-3-eCFP 和 CMV-eGFP质粒后, 给予神经生长因子(nerve growth factor, NGF)诱导, 观察绿色荧光蛋白和青色荧光蛋白的表达和分布。

结果: Axl-eGFP和Tyro-3-eCFP的表达随着NGF作用时间的延长而逐渐上调, 并且荧光蛋白在细胞内的定位也发生变化。 作为对照组, CMV-eGFP 转染的PC12 细胞并没有出现此变化。 此外, 过表达Axl 和Tyro-3 能够促进PC12 细胞的突起生长。

结论: Axl 和Tyro-3 受体的表达受NGF 调控, 其过表达可能参与了PC12 细胞的分化。

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