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. 1978 Sep;75(9):4291-5.
doi: 10.1073/pnas.75.9.4291.

Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro

M S Horwitz

Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro

M S Horwitz. Proc Natl Acad Sci U S A. 1978 Sep.

Abstract

A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.

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