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. 2011 Jan 28;16(2):1310-22.
doi: 10.3390/molecules16021310.

Triterpene composition and bioactivities of Centella asiatica

Puziah Hashim  1 , Hamidah Sidek, Mohd Helme M Helan, Aidawati Sabery, Uma Devi Palanisamy, Mohd Ilham
Affiliations

Triterpene composition and bioactivities of Centella asiatica

Puziah Hashim et al. Molecules. .

Abstract

Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC-PAD on an Excil ODS 5 mm (C18) column for the simultaneous determination of asiatic acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside (1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella (84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic activity was observed by the release of glycerol (115.9 µmol/L) at 0.02% concentration. Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. In view of these results, the potential application of Centella in food and pharmaceutical industries is now widely open.

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Figures

Figure 1
Figure 1
Triterpenes of Centella.
Figure 2
Figure 2
Stimulation of collagen synthesis of fibroblast cells using different concentration of Centella extracts (mg/mL) and vitamin C (25 µg/mL) was used as positive control. Untreated solvent was used as control. All fibroblast were incubated at 5% CO2 for 48 h incubation. Values are the mean ± SD (n = 3). Values with different letter are significant different at P < 0.05.
Figure 3
Figure 3
DPPH-radical scavenging activity of centella extract, grape seed, green tea and vitamin C at 1 mg/mL concentrations. Values are the mean ± SD (n = 3). Among the extracts studies, green tea shows the highest inhibition effect followed by vitamin C, Centella and grape seed extract. Values with different letter are significantly different at P < 0.05.
Figure 4
Figure 4
Effect of Centella extract and caffeine on lipolysis activity of adipocytes. Caffeine (1 mg/mL) was used as positive control and untreated solvent was used as control. The adipocytes with the positive control and test samples were incubated for 30 min at 37 °C with constant shaking in a CO2 incubator. Then infranatant was heated at 70 °C for 10 min and glycerol was measured using Randox kit. Values are the mean ± SD (n = 3).
Figure 5
Figure 5
The protection effect of Centella extract (CA), bearberry extract (BB) and octyl methoxy cinnamate (OMC) against UVA (ultraviolet-A) and UVB (ultraviolet-B) damage at different concentration; (a) at 0.1%, (b) at 1.0% and (c) at 10.0%. The 10% Centella extract shows comparable results with OMC.
Figure 5
Figure 5
The protection effect of Centella extract (CA), bearberry extract (BB) and octyl methoxy cinnamate (OMC) against UVA (ultraviolet-A) and UVB (ultraviolet-B) damage at different concentration; (a) at 0.1%, (b) at 1.0% and (c) at 10.0%. The 10% Centella extract shows comparable results with OMC.
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References

    1. Brinkhaus B., Lindner M., Schuppan D., Hahn E.G. Chemical, pharmacological and clinical profile of the East Asian medicinal plant Centella asiatica. Phytomedicine. 2000;7:427–448. doi: 10.1016/S0944-7113(00)80065-3. - DOI - PubMed
    1. James J.T., Dubery I.A. Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban. Molecules. 2009;14:3922–3941. doi: 10.3390/molecules14103922. - DOI - PMC - PubMed
    1. Tenni R., Zanaboni G., De Agustini M.P., Rossi A., Bendotti C., Cetta G. Effect of the triterpenoids fraction of Centella asiatica on macromolecules of the connective matrix in human skin fibroblast cultures. Italian J. Biochem. 1988;36:69–77. - PubMed
    1. Bonte F., Dumas M., Chaudagne C., Meybeck A. Influence of asiatic acid, madecassic acid and asiaticoside on human collagen I synthesis. Planta Med. 1994;60:133–135. doi: 10.1055/s-2006-959434. - DOI - PubMed
    1. Maquart F.X., Chasting F., Simeon A., Brembaut P., Gillery P., Wegrosky Y. Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds. Eur. J. Dermatol. 1999;9:289–296. - PubMed

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