Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts
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- PMID: 2128595
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Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts
Biochem Int.
1990 Oct.
Abstract
In this paper we report our studies on the effects of menadione in cultured fibroblasts treated with rotenone to block complex I. A normalization of the lactate to pyruvate ratio after incubation with glucose, an increased production of 14CO2 from [6-14C]glucose and an increased intra-cellular concentration of ATP was observed in the presence of micromolar concentrations of menadione. These results not only demonstrate the potential value of menadione in complex I deficient patients but also suggest that this system can be used advantageously for the in vitro assessment of therapeutic agents for disorders of the mitochondrial respiratory chain.
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