[Separation of enriched synaptosomes, synaptic vesicles and synaptic plasma membranes]
[Article in
German]
- PMID: 212909
Item in Clipboard
[Separation of enriched synaptosomes, synaptic vesicles and synaptic plasma membranes]
[Article in
German]
Acta Biol Med Ger.
1978.
Abstract
A rapid and simple method is described for separation of intact synaptosomes, synaptic plasma membranes and vesicles. Two synaptosome fractions were obtained by modified differential centrifugation. The rate zonal zentrifugation in a linear sucrose gradient (very low density) is suitable to obtain fractions highly enriched in synaptic plasma membranes and vesicles. Examination of the prepared fractions was done by enzyme marker activities and electron microscopy
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