Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Jan 25;18(2):323-9.
doi: 10.1093/nar/18.2.323.

Characterization of the promoter region of Tetrahymena genes

C F Brunk  1 , L A Sadler
Affiliations
Free PMC article
Comparative Study

Characterization of the promoter region of Tetrahymena genes

C F Brunk et al. Nucleic Acids Res. .
Free PMC article

Abstract

The regions between adjacent histone H3 and H4 genes, as well as portions of the genes, from 22 species of Tetrahymena have been amplified using the polymerase chain reaction and sequenced. Both histone genes are transcribed divergently with initiation occurring within the intergenic region, thus 2 sets of 22 homologous Tetrahymena promoters can be compared. A sequence comparison of these regions reveals a single putative promoter element, with a consensus sequence TATCCAATTCARA, present in front of each gene. This sequence contains a 'CCAAT' box, which also occurs at 8 locations preceding other ciliate genes. No other putative promoter sequences are found in front of these sets of histone genes. Sequences searched for include 'TATA' boxes, 'GC' boxes and other sequences suggested as putative promoter elements for ciliate genes. The coding strand immediately preceding ciliate genes is very high in A content and the consensus sequence at the site of protein synthesis is AAAATGG.

PubMed Disclaimer

References

    1. J Mol Biol. 1986 May 5;189(1):47-60 - PubMed
    1. Cell. 1986 Sep 12;46(6):795-805 - PubMed
    1. Cell. 1986 Jan 31;44(2):319-28 - PubMed
    1. Mol Cell Biol. 1987 May;7(5):1881-93 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5160-4 - PubMed

Publication types