Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar;90(3):331-5.
doi: 10.1177/0022034510386375.

Genetic diversity of plaque mutans streptococci with rep-PCR

K Cheon  1 , S A Moser, J Whiddon, R C Osgood, S Momeni, J D Ruby, G R Cutter, D B Allison, N K Childers
Affiliations

Genetic diversity of plaque mutans streptococci with rep-PCR

K Cheon et al. J Dent Res. 2011 Mar.

Abstract

Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
The number of isolates (n) estimated to be required to obtain a probability (p) of observing at least one of each hypothesized genotype (k) using the formula in the Power Calculations section of MATERIALS & METHODS.
Figure 2.
Figure 2.
Representative rep-PCR results from 10 individuals who had 20 isolates per individual analyzed. Virtual gel images represent DNA fingerprint patterns of MS isolates that were amplified with the automated rep-PCR system analyzed with DiversiLab software, version 3.3. The numbers identify individuals who participated in this study.
See this image and copyright information in PMC

References

    1. Alam S, Brailsford SR, Whiley RA, Beighton D. (1999). PCR-based methods for genotyping viridans group streptococci. J Clin Microbiol 37:2772-2776 - PMC - PubMed
    1. Bou G, Cervero G, Dominguez MA, Quereda C, Martinez-Beltran J. (2000). PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii. Clin Microbiol Infect 6:635-643 - PubMed
    1. Emanuelsson IR, Li Y, Bratthall D. (1998). Genotyping shows different strains of mutans streptococci between father and child and within parental pairs in Swedish families. Oral Microbiol Immunol 13:271-277 - PubMed
    1. Gold OG, Jordan HV, Van Houte J. (1973). A selective medium for Streptococcus mutans. Arch Oral Biol 18:1357-1364 - PubMed
    1. Healy M, Huong J, Bittner T, Lising M, Frye S, Raza S, et al. (2005). Microbial DNA typing by automated repetitive-sequence-based PCR. J Clin Microbiol 43:199-207 - PMC - PubMed

Publication types

LinkOut - more resources