Complete genome sequence of Acidimicrobium ferrooxidans type strain (ICP)

Abstract

Acidimicrobium ferrooxidans (Clark and Norris 1996) is the sole and type species of the genus, which until recently was the only genus within the actinobacterial family Acidimicrobiaceae and in the order Acidomicrobiales. Rapid oxidation of iron pyrite during autotrophic growth in the absence of an enhanced CO(2) concentration is characteristic for A. ferrooxidans. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of the order Acidomicrobiales, and the 2,158,157 bp long single replicon genome with its 2038 protein coding and 54 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

Keywords: Acidomicrobiales; Moderate thermophile; acidophile; ferrous-iron-oxidizing.

Figure 1

Phylogenetic tree highlighting the position of A. ferrooxidans strain ICP^T relative to all other type strains within the Acidimicrobiales and the type strains of all other orders within the Actinobacteria. The tree was inferred from 1306 aligned characters [4,5] of the 16S rRNA gene under the maximum likelihood criterion [6] and rooted with Rubrobacteriales. The branches are scaled in terms of the expected number of substitutions per site. Numbers above branches are support values from 1000 bootstrap replicates if larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [7] are shown in blue, published genomes in bold.

Figure 2

Scanning electron micrograph of A. ferrooxidans ICP^T (Manfred Rohde, Helmholtz Centre for Infection Research, Braunschweig)

Figure 3

Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.