Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Complete genome sequence of Brachyspira murdochii type strain (56-150)

Amrita Pati et al. Stand Genomic Sci. .

Abstract

Brachyspira murdochii Stanton et al. 1992 is a non-pathogenic, host-associated spirochete of the family Brachyspiraceae. Initially isolated from the intestinal content of a healthy swine, the 'group B spirochaetes' were first described as Serpulina murdochii. Members of the family Brachyspiraceae are of great phylogenetic interest because of the extremely isolated location of this family within the phylum 'Spirochaetes'. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of a type strain of a member of the family Brachyspiraceae and only the second genome sequence from a member of the genus Brachyspira. The 3,241,804 bp long genome with its 2,893 protein-coding and 40 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Keywords: Brachyspiraceae; GEBA; Gram-negative; Spirochaetes; anaerobic; host-associated; motile; non-pathogenic.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Phylogenetic tree highlighting the position of B. murdochii 56-150T relative to the other type strains within the genus and to the type strains of the other genera within the class Spirochaetes (excluding members of the Spirochaetaceae). The tree was inferred from 1,396 aligned characters [21,22] of the 16S rRNA gene sequence under the maximum likelihood criterion [23] and rooted in accordance with the current taxonomy. The branches are scaled in terms of the expected number of substitutions per site. Numbers above branches are support values from 1,000 bootstrap replicates if [24] larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [25] are shown in blue, published genomes in bold.
Figure 2
Figure 2
Scanning electron micrograph of B. murdochii 56-150T
Figure 3
Figure 3
Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.
See this image and copyright information in PMC

References

    1. Stanton TB, Fournie-Amazouz E, Postic D, Trott DJ, Grimont PAD, Baranton G, Hampson DJ, Saint Girons I. Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. Int J Syst Bacteriol 1997; 47:1007-1012 10.1099/00207713-47-4-1007 - DOI - PubMed
    1. Lee JI, Hampson DJ. Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol 1994; 40:365-371 10.1099/00222615-40-5-365 - DOI - PubMed
    1. Hampson DJ, La T. Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov. and Brachyspira murdochii comb. nov. Int J Syst Evol Microbiol 2006; 56:1009-1012 10.1099/ijs.0.64004-0 - DOI - PubMed
    1. Euzéby JP. List of bacterial names with standing in nomenclature: A folder available on the Internet. Int J Syst Bacteriol 1997; 47:590-592 10.1099/00207713-47-2-590 - DOI - PubMed
    1. Hovind-Hougen K, Birch-Andersen A, Henrik-Nielsen R, Orholm M, Pedersen JO, Teglbjaerg PS, Thaysen EH. Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J Clin Microbiol 1982; 16:1127-1136 - PMC - PubMed