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. 2011 Jan 31;6(1):e16388.
doi: 10.1371/journal.pone.0016388.

Landscape mapping of functional proteins in insulin signal transduction and insulin resistance: a network-based protein-protein interaction analysis

Chiranjib Chakraborty  1 , Sanjiban S Roy, Minna J Hsu, Govindasamy Agoramoorthy
Affiliations

Landscape mapping of functional proteins in insulin signal transduction and insulin resistance: a network-based protein-protein interaction analysis

Chiranjib Chakraborty et al. PLoS One. .

Abstract

The type 2 diabetes has increased rapidly in recent years throughout the world. The insulin signal transduction mechanism gets disrupted sometimes and it's known as insulin-resistance. It is one of the primary causes associated with type-2 diabetes. The signaling mechanisms involved several proteins that include 7 major functional proteins such as INS, INSR, IRS1, IRS2, PIK3CA, Akt2, and GLUT4. Using these 7 principal proteins, multiple sequences alignment has been created. The scores between sequences also have been developed. We have constructed a phylogenetic tree and modified it with node and distance. Besides, we have generated sequence logos and ultimately developed the protein-protein interaction network. The small insulin signal transduction protein arrangement shows complex network between the functional proteins.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Insulin signal transduction pathway.
The damage of insulin signal transduction causes insulin resistance, which is one of the main causes for type 2 diabetes.
Figure 2
Figure 2. Alignment scores of protein sequences related to insulin resistance pathways.
(A) Alignment score between sequences (notation Seq (x:y) meaning alignment score between sequence x, and sequence y), (B) scatter distribution of scores, and (C) scores connected by smoothed line without marker.
Figure 3
Figure 3. Phylogenetic tree construction using ClustalW.
Figure 4
Figure 4. Distance of the phylogenetic tree of 12 nodes (developed by MATLAB 7.3).
Figure 5
Figure 5. Ribbon like structure of the main functional proteins associated with pathway.
Collected from Protein Data Bank.
Figure 6
Figure 6. Main proteins associated with insulin signaling pathway.
Using WebLogo.
Figure 7
Figure 7. Protein-protein network in insulin signal transduction pathway.
See this image and copyright information in PMC

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