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. 2010 Dec;18(6):635-40.
doi: 10.1590/s1678-77572010000600017.

Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

Leandro Araújo Fernandes  1 , Thiago Marchi Martins, Juliano Milanezi de Almeida, Maria José Hitomi Nagata, Leticia Helena Theodoro, Valdir Gouveia Garcia, Alvaro Francisco Bosco
Affiliations

Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

Leandro Araújo Fernandes et al. J Appl Oral Sci. 2010 Dec.

Abstract

Objective: The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats.

Material and methods: In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05).

Results: In the histometric analysis, at 7, 15 and 30 days, Group T (0.72 ± 0.05 mm², 0.57 ± 0.14 mm², 0.62 ± 0.07 mm²), showed less bone loss (p<0.05) than Group C (1.35 ± 0.25 mm²; 1.40 ± 0.31 mm²; 1.29 ± 0.27 mm²), respectively.

Conclusions: Subgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats.

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Figures

Figure 1
Figure 1
Experimental design
Figure 2
Figure 2
Photomicrograph illustrating bone loss area in the furcation region of mandibular first molar in the initial periods and in the different Groups (control and tetracycline-HCL): (A) Control Group – 30 days – middle third into the furcation region (Original magnification x12.5) – Detail (B) Note the thin bone trabeculae with resorption activity tissue (Original magnification x40); (C) tetracycline-HCL Group - 30 days – coronary third into the furcation region (Original magnification x12.5) – Detail (D) Note thick bone trabeculae without signs of resorption (Original magnification x40) (Hematoxylin and Eosin)
Figure 3
Figure 3
Photomicrograph illustrating bone tissue in the furcation region of mandibular first molar in the different Groups (control and tetracycline-HCL) and Periods (7, 15, 30 days): (A) Control group - 7 days; (B) Tetracycline-HCL group - 7 days; (C) Control group – 15 days; (D) Tetracycline-HCL group – 15 days; (E) Control group – 30 days; (F) Tetracycline-HCL group – 30 days (Original magnification x12.5; Masson’s Trichrome)
See this image and copyright information in PMC

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