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. 2011 Aug;91(8):728-36.
doi: 10.1002/bdra.20770. Epub 2011 Feb 9.

Racial differences in gene-specific DNA methylation levels are present at birth

Ronald M Adkins  1 , Julia Krushkal, Frances A Tylavsky, Fridtjof Thomas
Affiliations

Racial differences in gene-specific DNA methylation levels are present at birth

Ronald M Adkins et al. Birth Defects Res A Clin Mol Teratol. 2011 Aug.

Abstract

Background: DNA methylation patterns differ among children and adults and play an unambiguous role in several disease processes, particularly cancers. The origin of these differences is inadequately understood, and this is a question of specific relevance to childhood and adult cancer.

Methods: DNA methylation levels at 26,485 autosomal CpGs were assayed in 201 newborns (107 African American and 94 Caucasian). Nonparametric analyses were performed to examine the relation between these methylation levels and maternal parity, maternal age, newborn gestational age, newborn gender, and newborn race. To identify the possible influences of confounding, stratification was performed by a second and third variable. For genes containing CpGs with significant differences in DNA methylation levels between races, analyses were performed to identify highly represented gene ontological terms and functional pathways.

Results: 13.7% (3623) of the autosomal CpGs exhibited significantly different levels of DNA methylation between African Americans and Caucasians; 2% of autosomal CpGs had significantly different DNA methylation levels between male and female newborns. Cancer pathways, including four (pancreatic, prostate, bladder, and melanoma) with substantial differences in incidence between the races, were highly represented among the genes containing significant race-divergent CpGs.

Conclusions: At birth, there are significantly different DNA methylation levels between African Americans and Caucasians at a subset of CpG dinucleotides. It is possible that some of the epigenetic precursors to cancer exist at birth and that these differences partially explain the different incidence rates of specific cancers between the races.

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Figures

Figure 1
Figure 1
Venn diagrams summarizing the overlap in genomewide statistically significant CpG methylation differences when (A) newborn gender, (B) maternal age (tertiles), or (C) newborn race are further stratified by an additional one or two variables and analyzed by the Kruskal-Wallis rank sum test as described by van Elteren (1960).
See this image and copyright information in PMC

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