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. 1978 Oct 16;90(3):581-3.
doi: 10.1111/j.1432-1033.1978.tb12638.x.

Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence

U Günthert, K Storm, R Bald
Free article

Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence

U Günthert et al. Eur J Biochem. .
Free article

Abstract

Calf thymus DNA was methylated in vitro with cell extracts of Bacillus subtilis OG3R (r+m+) and S-adenosyl[Me-3H]methionine. After depurination of the [3H]methylated DNA, the analysis of the pyrimidine dinucleotides revealed the following positions of the methylated nucleosides (indicated by an asterisk) within the BsuRI recognition sequence: 5' dG--dG--dC--dC dC--dC--dG--dG 5'.

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