Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;1(1):73-8.
doi: 10.3109/10425179009041349.

Semiautomated preparation of DNA templates for large-scale sequencing projects

V Smith  1 , C M Brown, A T Bankier, B G Barrell
Affiliations

Semiautomated preparation of DNA templates for large-scale sequencing projects

V Smith et al. DNA Seq. 1990.

Abstract

The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.

PubMed Disclaimer

Publication types