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. 2010 Dec;3(4):132-6.
doi: 10.2478/v10102-010-0053-8.

Pycnogenol and Ginkgo biloba extract: effect on peroxynitrite-oxidized sarcoplasmic reticulum Ca-ATPase

Petronela Zižková  1 , Jana Viskupičová, L'ubica Horáková
Affiliations

Pycnogenol and Ginkgo biloba extract: effect on peroxynitrite-oxidized sarcoplasmic reticulum Ca-ATPase

Petronela Zižková et al. Interdiscip Toxicol. 2010 Dec.

Abstract

The effect of two natural standardized plant extracts, Pycnogenol(®) and EGb 761, on sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) activity and posttranslational modifications induced by peroxynitrite was investigated to assess their possible protective role. EGb 761 was found to have a protective effect on SERCA activity in the concentration range of 5-40 µg/ml. On the other hand, Pycnogenol(®) caused a decrease of SERCA activity at concentrations of 25 µg/ml. EGb 761 did not prevent protein carbonyl formation upon oxidation with peroxynitrite. On the contrary, Pycnogenol(®) at the concentrations of 5 and 10 µg/ml significantly decreased the level of protein carbonyls by 44% and 54%, respectively. Neither Pycnogenol(®) nor EGb 761 exerted a protective effect against thiol group oxidation.The plant extracts studied modulated peroxynitrite-injured SERCA activity by different ways and failed to correlate with posttranslational modifications. Their effect seems to be associated with their ability to change SERCA conformation rather than by their antioxidant capacity.

Keywords: Ca2+-ATPase; EGb 761; Pycnogenol®; peroxynitrite.

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Figures

Figure 1
Figure 1
Effect of EGb 761 on SERCA activity in the presence of ONOO– (100 µM). SR was exposed to various concentrations of EGB 761 for 2 min followed by oxidation by peroxynitrite at 25°C, pH 7.2. Values are mean ± SD of two independent experiments, with each sample measured in three parallels. ***p<0.001 are significant differences between oxidized sample and EGB 761 treated samples.
Figure 2
Figure 2
Effect of Pycnogenol® on SERCA activity in the presence of ONOO– (100 µM). SR was exposed to various concentrations of Pycnogenol® for 2 min followed by oxidation with peroxynitrite (25°C, pH 7.2). Values are mean ± SD of three independent experiments, with each sample measured in two parallels.**p<0.01 are significant differences between oxidized sample and Pycnogenol® treated samples.
Figure 3
Figure 3
Production of protein carbonyls in pretreament of SERCA with EGb 761 and Pycnogenol® in the presence of peroxynitrite. Values are mean ± SD of three independent experiments, with each sample measured in four parallels. ***p<0.0001 are significant differences between oxidized sample and flavonoid treated samples.
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