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. 2011 Feb;26(1):40-6.
doi: 10.1007/s12250-011-3162-9. Epub 2011 Feb 18.

Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA

Zhe Ren  1 , Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui, Yi-fei Wang, Ren-bin Qi, Da-xiang Lu, Shu-min Zhang, Pei-zhuo Zhang
Affiliations

Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA

Zhe Ren et al. Virol Sin. 2011 Feb.

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

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