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. 2011 Feb;26(1):40-6.
doi: 10.1007/s12250-011-3162-9. Epub 2011 Feb 18.

Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA

Zhe Ren  1 , Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui, Yi-fei Wang, Ren-bin Qi, Da-xiang Lu, Shu-min Zhang, Pei-zhuo Zhang
Affiliations

Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA

Zhe Ren et al. Virol Sin. 2011 Feb.

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

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References

    1. Andino R. RNAi puts a lid on virus replication. Nat Biotechnol. 2003;21:629–630. doi: 10.1038/nbt0603-629. - DOI - PubMed
    1. Bhuyan P. K., Kariko K., Capodici J. Short interfering RNA-mediated inhibition of herpes simplex virus type 1 gene expression and function during infection of human keratinocytes. J Virol. 2004;78:10276–10281. doi: 10.1128/JVI.78.19.10276-10281.2004. - DOI - PMC - PubMed
    1. Brandt C. R., Kintner R. L., Pumfrey A. M., et al. The herpes simplex virus ribonucleotide reductase is required for ocular virulence. J Gen Virol. 1991;72:2043–2049. doi: 10.1099/0022-1317-72-9-2043. - DOI - PubMed
    1. Cameron J. M., Mcdougall I., Marsden H. S., et al. Ribonucleotide reductase encoded by herpes simplex virus is a determinant of the pathogenicity of the virus in mice and a valid antiviral target. J Gen Virol. 1988;69:2607–2612. doi: 10.1099/0022-1317-69-10-2607. - DOI - PubMed
    1. Cory J. G. Ribonucleotide reductase as a chemotherapeutic target. Adv Enzyme Regul. 1988;27:437–455. doi: 10.1016/0065-2571(88)90030-1. - DOI - PubMed

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